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FREEZE CONTROL BIOFREEZER TECHNOLOGY

FREEZE CONTROL BIOFREEZER TECHNOLOGY

Biogenics offers a complete line of FREEZE CONTROL Biofreezer systems, precision engineered for cryopreservation of biological specimens. FREEZE CONTROL systems provide a patented and reliable method for heat transfer and temperature regulation in the freezing of biological material for long-term preservation and viable recovery.

These controlled-rate, non-pressurized liquid nitrogen systems include a FREEZE CONTROL temperature controller, a straw cryochamber and a cryobath. Options in cryochambers and cryobaths are available—Biogenics will work with you to determine the most appropriate combination for your specific application requirements.

PreProgrammed Models
(CL2200, CL5500, CL8800)

The customer may specify target temperatures, rates, and holds on preprogrammed temperature controller models. Sets of programs are written and stored onto a single memory "chip", which is easily user-replaceable. Final program state can be "Hold temperature at finish" or "Freefall at finish". If the customer requires future program changes, a replacement program chip can be provided. Preprogrammed mode offers a level of increased security, because the programs cannot be inadvertently deleted or modified.

User-Programmable Models
(CL3300, CL8800)

CryoGenesis™ Version 5 temperature control software is included with user-programmable models. This Windows™-based software allows the user to design temperature protocols by generating a set of ramps (including target temperatures, rates, holds, and alarms); manage protocol libraries (save, load, edit); execute protocols (use real time data, allow user interactions); log progress and allow for clinical validation documentation. As with preprogrammed models, final program state may be "Hold" or "Freefall" to the temperature of LN2.

 

 

FREEZE CONTROL®, Temperature Controller Programming
Sample freezing protocols for clinical and multi-species applications:
Application Rate (°C/min.) Target Temp (°C) Hold Time (min.)
Clinical: Start Temp: 20.0°C   Final State: Hold
Modified Testarte 2.00 -6.0 10.00
  0.30 -35.0 0.00
  Start Temp: 18.0°C   Final State: Hold
Blastocyst, after 1.00 -7.0 10.00
Manezzo et al 0.03 -35.0 0.00
Equine: Start Temp: 24.0°C   Final State: Free Fall
Semen, after 4.00 18.00 0.00
McKinnon et al 0.10 6.00 10.00
(Seeding Plateau) 2.00 -6.00 3.0
  6.00 -30.00 Free Fall
Bovine: Start Temp: -6.0°C   Final State: Hold
Embryo, Standard 0.00 -6.00 10.00
Glycerol 0.50 -32.0 0.00
  Start Temp: -7°C   Final State: Hold
Embryo, Standard 0.00 -7.0 10.00
Ethylene Glycol 0.50 -35.0 0.00

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